Sei cryovials inoputika?Nzira yekuzvidzivirira sei?

Sei cryovials inoputika?Nzira yekuzvidzivirira sei?

Panguva yekuedza, tinogona kushandisacryovialskuomesa sampuli, asi kana kuchitonhora ne nitrogen yemvura,cryovialskazhinji kuputika, izvo zvisingangokonzeresa kurasikirwa kwemasampuli ekuyedza, asi zvinogona kutokonzera kukuvadzwa kwemasampuli.Vanoedza vanokonzeresa kukuvadza, saka ungadzivirira sei izvi kuti zvisaitike?

Zvikonzero:

Kutanga kwazvose,cryovialshaigoni kuiswa zvakananga muchikamu chemvura yenitrogen yemvura kuitira kuchengetedza.Nekuti zvinhu zvechubhu muviri uye kavha yezvakajairikacryovialszvakasiyana, mazinga ekuwedzera kwekupisa uye kuputika panguva yechando zvakasiyanawo.Kana iwe ukaisa iyocryovialzvakananga muchikamu chemvura, unogona kubvumira mvura nitrogen kuyerera muchubhu.Paunenge uchidzorera sampuli nguva inotevera, isa iyocryogenic-midziyomubhati remvura pa37 ° C, iyo nitrogen yemvura muchubhu yakakurumidza kunyungudika uye yakawedzera, asi gasi harina kukwanisa kubuda kubva muchubhu munguva, izvo zvakaita kuti cryopreservation chubhu iputika.

Nzira yekudzivisa:

1. Usachengetacryovialszvakananga muchikamu chemvura, asi muchikamu chegesi.Kana kungoisa mufiriji zvakananga.Rangarira kusaiisa pazasi pepamusoro pemvura yenitrogen.

2. Shandisa kutenderera kwemukaticryotubes.

Zvechokwadi, kunyange mukati kutendereracryotubeshaigone kuiswa yakananga muchikamu chemvura, asi mukati inotenderedzwacryotubeskuve nekushivirira kurinani kwekushisa kupfuura zvivharo zvinotenderedzwa nekunze, izvo zvinogona kuderedza mukana wekuputika uye zvakachengetedzeka.Kutenderera kwekunzecriyotubeyakanyatsogadzirirwa kuomesa nechando, saka haina kukodzera kuchengetwa kwenitrogen yemvura.

3. Saka chii chaunofanira kuita kana iwe uchida chaizvo kuichengeta muchikamu chemvura?Kupindura dambudziko iri, pane chaizvo cryopreservation chubhu maoko akagadzirirwa chinangwa ichi, anogona kushandiswa kunama cryopreservation chubhu uye woiisa muchikamu chemvura.Zvechokwadi, iwe unogonawo kushandisa kuvhara firimu, tepi yezvokurapa, nezvimwewo kuti uisimbise, kuitira kuti pave pasina kuputika.