Shandisa Nzira uye Chengetedzo yeKupisa Tube
Muzviedzo zvemicrobiological, imwe midziyo yekuyedza inowanzoshandiswa, kureva, cryopreservation chubhu.Nekudaro, nekuda kwekuoma kwavo kwakasiyana, mhedzisiro inosiyana zvakanyanya.Nokuda kwechikonzero ichi, pari zvino, marabhoritari akawanda muChina anoita mabhakitiriya ekuchengetedza machubhu ega, izvo zvisingangowedzere kushanda nesimba, asiwo nekuda kwekukanganisa kwemamiriro ezvinhu akasiyana-siyana, mhedzisiro yekuchengetedzwa kwebhakitiriya haisi nguva dzose inogutsa.
Naizvozvo, zvinodikanwa kugona nzira yekushandisa uye dzimwe dziviriro dzeiyo cryopreservation chubhu, kuitira kuti uite basa rakakura.
1.Method yekushandisa
1).Paunenge uchishandisa cryopreservation chubhu kuchengetedza samples, zvinonyatsodikanwa kuisa cryopreservation chubhu mumhute yemvura nitrogen kana firiji yekuchengetera.Kana iyo cryopreservation chubhu yakachengetwa mumvura ine nitrogen, pane imwe mukana wekuti iyo nitrogen yemvura inopinda muchubhu ye cryopreservation.Munguva yekupora, iyo gasification yemvura nitrogen inotungamira mukusaenzana kwemukati nekunze kudzvanywa, izvo zvingangoita kuti cryopreservation chubhu iparare, uye ine biological njodzi.
2).Shanda iyo cryopreservation chubhu kumutsidzira, uye shandisa midziyo yekuchengetedza kuchengetedza mukati mekuita.Zvinokurudzirwa kupfeka zvipfeko zverabhoritari, magurovhosi ekotoni uye kushanda pabhenji rakachengeteka re laboratori.Kana zvichibvira, ndapota pfeka magogi kana kuti face shield.Sezvo tembiricha yemukati muzhizha ichave yakakwirira kupfuura iyo munguva yechando, ndapota chenjera.
3).Munguva yekuchengetwa kwemasero e-cryopreserved, kutonhora kwechando kwe cryopreserved tubes kunofanira kunge kwakafanana.Kutonhora zvisina kuenzana kunotungamira kune jam yechando, iyo inodzivirira kutapurirana kwemvura tembiricha pamativi ese, nekudaro zvichiunza njodzi yakanyanya kudzvanywa uye kukonzera kukuvadza kune inotonhora chubhu.
4).Huwandu hwemasamples akaomeswa nechando hahufanire kudarika huwandu hwekushanda hunodiwa nechubhu yechando.
2. Nyaya dzinoda kutarisirwa
1).Kutonhora kwechubhu yekuchengetedza nzvimbo
Cryopreservation tubes isina kushandiswa inogona kuchengetwa mukamuri tembiricha kana 2-8 ℃ kwemwedzi gumi nemiviri;Iyo inoculated cryopreservation chubhu inogona kuchengetwa pa - 20 ℃ uye ine mhedzisiro yakanaka yekuchengetedza kushungurudza mukati memwedzi gumi nemaviri;Iyo inoculated cryopreservation chubhu inogona kuchengetwa pa - 80 ℃, uye kuomarara kunogona kuchengetedzwa zvakanaka mukati memwedzi makumi maviri nemana.
2).Kutonhora kwechubhu kuchengetedza nguva
Isina kushandiswa cryopreservation tubes inogona kuchengetwa pakamuri tembiricha kana 2-8 ℃;Iyo inoculated cryopreservation chubhu ichachengetwa pa - 20 ℃ kana - 80 ℃.
3).Operation matanho echando chubhu
Tora hutsva hutsva kubva kutsika dzakachena dzebhakitiriya kuti ugadzirire kumiswa kwebhakitiriya ne turbidity inosvika 3-4 McDonnell's ratio yeinoculation uye strain preservation chubhu;Simbisa chubhu yekuchengetedza uye uidzorere kumashure uye mberi 4-5 nguva kuita kuti mabhakitiriya emulsified, pasina kutenderera;Isa iyo yekuchengetedza chubhu mufiriji kuti ichengetedze (- 20 ℃ - 70 ℃
Nguva yekutumira: Nov-25-2022