Mazano ekusarudza uye kushandiswa kwesero tsika plate (I)
Sechishandiso chakajairwa uye chakakosha chesero tsika, sero tsika ndiro ine akasiyana maumbirwo, zvakatemwa uye mashandisiro.
Iwe zvakare wakavhiringika nezve nzira yekusarudza iyo chaiyo tsika ndiro?
Uri kunetsekana nezve mashandisiro etsika ndiro zviri nyore uye nemazvo?
Uri kuvhiringika nezve maitiro ekuita netsika ndiro?
Iwe unonzwa sei nezve kushandiswa kunoshamisa kweakasiyana tsika ndiro?
Nzira yekusarudza cell culture plate?
1) Iyo sero tsika mahwendefa anogona kukamurwa kuita sandara pasi uye kutenderera pasi (U-yakaumbwa uye V-yakaumbwa) zvinoenderana nechimiro chepasi;
2) Nhamba yemakomba etsika yaive 6, 12, 24, 48, 96, 384, 1536, zvichingodaro;
3) Zvinoenderana nemhando dzakasiyana siyana, kune Terasaki ndiro uye yakajairwa cell tsika ndiro.Sarudzo chaiyo inoenderana nerudzi rwemaseru ane tsika, inodiwa tsika vhoriyamu uye akasiyana ekuedza zvinangwa.
Musiyano uye kusarudzwa kwepasi uye kutenderera pasi (U-yakaita uye V-yakaumbwa) mahwendefa etsika
Mhando dzakasiyana dzemapuranga zvinowanzove nekushandiswa kwakasiyana
Mhando dzose dzemaseru epasi pasi anogona kushandiswa, asi kana nhamba yemasero idiki, senge cloning, 96 tsime rezasi mahwendefa anoshandiswa.
Pamusoro pezvo, kana uchiita MTT uye zvimwe zviedzo, iyo furati yepasi ndiro inowanzo shandiswa kune ese anonamatira uye akamiswa maseru.
Kana ari mahwendefa ane U-akaumbwa kana V, anowanzo shandiswa mune zvimwe zvakakosha zvinodiwa.Semuenzaniso, mune immunology, kana maviri akasiyana lymphocytes akasanganiswa, anofanirwa kusangana kune mumwe nemumwe kuti akurudzire.Naizvozvo, mahwendefa akaita U-anowanzo kudiwa.Nekuti maseru achaungana mudiki nekuda kwekuita kwesimba rinokwevera zvinhu pasi, mahwendefa ane chimiro cheV haanyanyi kubatsira.Mahwendefa akafanana neV anowanzo shandiswa mukuedza kuuraya masero kuti aite masero ekunangwa akanyatsobatana, asi mahwendefa ane U-anogona kushandiswa mukuedza uku (mushure mekuwedzera masero, centrifuge pakaderera).
Kana yakashandiswa sero tsika, inowanzoita flat bottomed.Mukuwedzera, kunyanya kukoshesa kunofanira kubhadharwa kune zvinyorwa.Mucherechedzo wekuti "Tissue Tsika (TC) Yakabatwa" inoshandiswa kune sero tsika.
Iyo yakatenderedza pasi inowanzo shandiswa pakuongorora, kemikari reaction, kana sampuli kuchengetedza.Nekuti denderedzwa zasi zvirinani kunwisa zvinwiwa, uye flat bottoms hazvisi.Nekudaro, kana iwe uchida kuyera kukosha kwekunyudza kwemwenje, unofanirwa kutenga yepasi pasi.
Mazhinji masero tsika anoshandisa flat pasi tsika mahwendefa, ayo ari nyore kuona pasi pemaikorosikopu, ane nzvimbo yepasi yakajeka, ane inowirirana sero tsika yemvura kureba, uye zvakare kufambisa kuonekwa kweMTT.
Iyo yakatenderedza yepasi tsika ndiro inonyanya kushandiswa kuyedza yekubatanidzwa isotopu, uye sero yekuunganidza chiridzwa chinodiwa kuunganidza sero tsika, senge "musanganiswa lymphocyte tsika".
Nguva yekutumira: Zvita-08-2022