Nhanho yekutanga kune yakabudirira ELISA kuyedza-kusarudza yakakodzera ELISA ndiro

TheELISAndiro chishandiso chakakosha cheELISA, iyo enzyme-yakabatana immunosorbent assay.Pane zvinhu zvakawanda zvinokanganisa budiriro yeELISA kuyedza.Kusarudza chishandiso chakakodzera ndiro danho rekutanga.Kusarudza microplate yakakodzera kunobatsira kuedza kubudirira.

Zvinhu zveELISAndiro inowanzoita polystyrene (PS), uye polystyrene haina kugadzikana kwemakemikari uye inogona kunyungudutswa nemhando dzakasiyana-siyana dzezvinonyungudutsa (sezvinonhuhwirira hydrocarbons, halogenated hydrocarbons, nezvimwewo), uye inogona kusvibiswa nemaasidhi akasimba uye alkalis.Isingamirire girizi uye kushanduka nyore nyore mushure mekuratidzwa kune UV mwenje.

Ndeapi marudzi eELISAndiro dziripo?

✦Sarudza nemuvara

Transparent plate:akakodzera kuwanda uye qualitative solid-phase immunoassays uye anosunga assays;

White plate:inokodzera self-luminescence uye chemiluminescence;

Black plate:yakakodzera fluorescent immunoassays uye anosunga assays.

✦Sarudza nekusunga simba

Ndiro yekusunga yakaderera:Kungosunga chete kumapuroteni kuburikidza nepamusoro pe hydrophobic bond.Inokodzera seyakasimba-chikamu chinotakura macromolecular mapuroteni ane huremu hwemamorekuru> 20kD.Mapuroteni ayo anosunga simba ndeye 200 ~ 300ng IgG/cm2.

High binding plate:Mushure mekurapwa kwepamusoro, simba rayo rekusunga mapuroteni rinokwidziridzwa zvakanyanya, richisvika 300 ~ 400ng IgG/cm2, uye huremu hwemamorekuru hweprotein yakasungwa ndeye> 10kD.

✦ Ronga nepasi chimiro

Pazasi pasi:low refractive index, yakakodzera kuonekwa ne microplate readers;

U pasi:Iyo refractive index yakakwira, iyo yakanakira kuwedzera, kushuvira, kusanganisa uye mamwe mabasa.Iwe unogona kuona zvakananga shanduko yeruvara nekutarisa kwekuona pasina kuiisa pane iyo microplate muverengi kuti uone kana paine inoenderana immune immune reaction.