Matanho Akananga eSero tsika

1. Zvishandiso zvakajairika

1. Zvishandiso muimba yekugadzirira

Single distilled water distiller, double distilled water distiller, acid tank, oveni, pressure cooker, kabati yekuchengetera (yekuchengeta zvinhu zvisina kugadzirwa), kabati yekuchengetera (yekuchengeta zvinhu zvisina sterilized), tafura yekurongedza.Zvishandiso mukamuri yekugadzirira mhinduro: torsion chiyero uye chiyero chemagetsi (mushonga wehuremu), PH mita (kuyera PH kukosha kwetsika mhinduro), magineti stirrer (kugadzirisa mhinduro mukamuri yekumutsa mhinduro).

2. Midziyo yekamuri yetsika

Liquid nitrogen tank, storage cabinet (kuchengetedza sundries), fluorescent rambi uye ultraviolet rambi, air purifier system, yakadzika tembiricha firiji (- 80 ℃), air conditioner, carbon dioxide cylinder, side table (kunyora bvunzo zvinyorwa).

3. Zvishandiso zvinofanira kuiswa muimba isina utachiona

Centrifuge (kuunganidza masero), ultra-clean worktable, inverted microscope, CO2 incubator (incubating tsika), kugeza mvura, matatu-oxygen disinfection uye sterilization machine, 4 ℃ firiji (kuisa serum uye tsika mhinduro).

2, Aseptic kushanda

(1) Sterilization yekamuri isina hutachiona

1. Chenesa imba isina tsvina nguva dzose: kamwe chete pavhiki, tanga kushandisa mvura yepombi kukorobha pasi, kupukuta tafura, uye kuchenesa tafura yekushanda, uye shandisa 3 ‰ lysol kana bromogeramine kana 0.5% peracetic acid kupukuta.

2. Sterilization ye CO2 incubator (incubator): tanga kupukuta ne 3 ‰ bromogeramine, wozopukuta ne 75% doro kana 0.5% peracetic acid, uye ipapo irradiate ne ultraviolet rambi.

3. Kuvharisa kusati kwaedza: batidza mwenje we ultraviolet, matatu-oxygen sterilizer uye air purifier system kwemaminitsi 20-30 zvakateerana.

4. Sterilization mushure mekuedza: pukuta tafura ye-ultra-yakachena, tafura yeparutivi uye inverted maikorosikopu padanho ne75% doro (3 ‰ bromogeramine).

Sterilization kugadzirira kwevashandi ve laboratori

1. Geza maoko nesipo.

2. Pfeka hembe dzekuzviparadzanisa nevamwe, makepesi ekuzviparadzanisa nevamwe, masiki nemaslippers.

3. Pukuta maoko ne75% doro donje bhora.

Kuratidzira kwekuita steril operation

1. Mabhodhoro ose edoro, PBS, tsika yepakati uye trypsin inounzwa mu-ultra-clean workbench inofanira kupukuta ne 75% doro pamusoro pekunze kwebhodhoro.

2. Shanda pedyo nemurambi wedoro.

3. Midziyo inofanira kucheneswa isati yashandiswa.

4. Midziyo (yakadai semabhodhoro ehombodo uye madonhwe) inoramba ichishandiswa inofanira kuiswa panzvimbo yakakwirira, uye inofanira kuramba ichipisa panguva yekushandiswa.

5. Zvose zvinoshandiswa zvinofanira kuva pedyo nemwenje wedoro, uye chiito chacho chinofanira kuva chakareruka uye chakarurama, uye hachifaniri kubatwa zvisina kufanira.Kana mashanga asingakwanise kubata tangi remarara.

6. Kana uchishuvira marudzi anopfuura maviri emvura, chenjerera kutsiva pombi yekusveta kudzivirira kusvibiswa kwemuchinjikwa.

Ona chitsauko chinotevera chekuuraya utachiona hwezviridzwa.