Iyo sero tsika mahwendefa anogona kukamurwa kuita sandara pasi uye kutenderera pasi (U-yakaumbwa uye V-yakaumbwa) zvinoenderana nechimiro chepasi;Huwandu hwemakomba etsika hwaive 6, 12, 24, 48, 96, 384, 1536, nezvimwe;Zvinoenderana neyakasiyana midziyo, kune Terasaki ndiro uye yakajairwa cell tsika ndiro.Sarudzo chaiyo inoenderana nerudzi rwemaseru ane tsika, inodiwa tsika vhoriyamu uye akasiyana ekuedza zvinangwa.
(1) Musiyano uye kusarudzwa kwepasi uye kutenderera pasi (U-yakaumbwa uye V-yakaumbwa) mahwendefa etsika
Zvimiro zvakasiyana zvetsika mahwendefa ane kushandiswa kwakasiyana.Masero etsika anowanzo kudzika pasi, izvo zviri nyore kutariswa nemicroscopic, ine nzvimbo yepasi yakajeka uye inowirirana sero tsika yemvura level.Naizvozvo, kana uchiita MTT uye zvimwe zviedzo, iyo yakadzika pasi ndiro inowanzo shandiswa, zvisinei nekuti maseru akasungirirwa kumadziro kana kumiswa.Iyo yakadzika pasi tsika ndiro inofanira kushandiswa kuyera kukosha kwekunyura.Nyatsoteerera kune izvo zvinyorwa, uye maka "Tissue Tsika (TC) Yakarapwa" yesero tsika.
Mahwendefa ane U-akaumbwa kana V anowanzo shandiswa mune zvimwe zvakakosha zvinodiwa.Semuenzaniso, mune immunology, kana maviri akasiyana lymphocytes akavhenganiswa netsika, anofanirwa kubata uye kukurudzirana.Panguva ino, mahwendefa akaita U-anowanzo shandiswa nekuti maseru anoungana mudiki nekuda kwekuita kwesimba rinokwevera zvinhu pasi.Iyo yakatenderedza pasi tsika ndiro inogona zvakare kushandiswa kuyedza yeisotope incorporation, iyo inoda kuti sero yekuunganidza chiridzwa kuunganidza sero tsika, senge "musanganiswa lymphocyte tsika".V-mahwendefa anowanzo shandiswa kuuraya masero uye immunological ropa agglutination bvunzo.Kuedza kwekuuraya masero kunogonawo kutsiviwa neU-shaped plate (mushure mekuwedzera masero, centrifuge pakumhanya kwakaderera).
(2) Misiyano pakati peTerasaki ndiro uye yakajairwa sero tsika ndiro
Terasaki ndiro inonyanya kushandiswa crystallographic research.Chigadzirwa dhizaini chakanakira kutarisa kwekristaro uye kuongororwa kwemaitiro.Pane nzira mbiri: kugara uye kurembera kudonha.Nzira mbiri idzi dzinoshandisa zvigadziro zvakasiyana zvechigadzirwa.Crystal kirasi polymer inosarudzwa sechinhu, uye zvakakosha zvinhu zvinogoneka pakuona chimiro chekristaro.
Iyo sero tsika ndiro inonyanya kugadzirwa nePS zvinhu, uye zvinhu zvacho zvinorapwa pamusoro, izvo zviri nyore kune sero adherent kukura uye kuwedzera.Ehezve, kune zvakare zvinhu zvekukura zvemaplanktonic masero, pamwe neakaderera anosunga pamusoro.
(3) Misiyano pakati pesero tsika ndiro neElisa ndiro
Iyo Elisa ndiro kazhinji inodhura kupfuura cell tsika ndiro.Iyo sero ndiro inonyanya kushandiswa sero tsika uye inogona zvakare kushandiswa kuyera mapuroteni ekugadzirisa;Iyo Elisa ndiro inosanganisira yekubikira ndiro uye reaction plate, uye kazhinji haidi kushandiswa sero tsika.Iyo inonyanya kushandiswa kuona mapuroteni mushure mekudzivirira enzyme-yakabatana maitiro, inoda yakakwirira zvinodiwa uye chaiyo enzyme label inoshanda mhinduro.
(4) Gomba pazasi nzvimbo uye yakakurudzirwa mvura dosi yeanowanzo shandiswa akasiyana tsika mahwendefa
Iyo yemvura mwero weiyo tsika yemvura yakawedzerwa kune akasiyana orifice mahwendefa haifanirwe kunge yakadzika zvakanyanya, kazhinji mukati meiyo 2 ~ 3mm.Iyo yakakodzera yemvura yakawanda yega yega tsika gomba inogona kuverengerwa nekubatanidza nzvimbo yepasi yemakomba akasiyana.Kana mvura yakawandisa ichiwedzerwa, gasi (oxygen) kuchinjana kuchakanganiswa, uye zviri nyore kupfachukira panguva yekufamba, zvichikonzera kusvibiswa.Iyo chaiyo cell density inoenderana nechinangwa chekuyedza.
Nguva yekutumira: Nov-04-2022