Sample kuunganidza, kuchengetedza uye chekufambisa zvinodiwa zvezviyedzo zvakajairika

Sample kuunganidza, kuchengetedza uye chekufambisa zvinodiwa zvezviyedzo zvakajairika

1. Kuunganidza uye kuchengetedzwa kwepathological samples:

☛Chikamu chakaomeswa nechando: Bvisa mabhuroko ematishu akakodzera woachengeta munitrogen yemvura;

☛Parafini chikamu: Bvisa matishu akakodzera uye achengete mu 4% paraformaldehyde;

☛Masiraidhi esero: Masiraidhi esero akaiswa mu4% paraformaldehyde kwemaminetsi makumi matatu, ndokutsiviwa nePBS ndokunyudzwa muPBS ochengetwa pa4°C.

2. Kuunganidzwa uye kuchengetedzwa kwemamolekyuli ebhayoloji sampuli:

☛Matishu matsva: Cheka mufananidzo uye uchengetedze munitrogen yemvura kana -80 ° C mufiriji;

☛Mienzaniso yeParafini: Chengeta patembiricha yemumba;

☛Muenzaniso weropa rose: Tora huwandu hwakakodzera hweropa rose uye wedzera EDTA kana heparin anticoagulation yekuunganidza ropa chubhu;

☛Masampuli emvura yemuviri: high-speed centrifugation kuunganidza sediment;

☛Masero emuenzaniso: Masero anoiswa lysed neTRizol uye anochengetwa mumvura ine nitrogen kana -80°C mufiriji.

3. Kuunganidza uye kuchengetwa kwemapuroteni ekuedza sampuli:

☛Matishu matsva: Cheka mufananidzo uye uchengetedze munitrogen yemvura kana -80 ° C mufiriji;

☛Muenzaniso weropa rose: Tora huwandu hwakakodzera hweropa rose uye wedzera EDTA kana heparin anticoagulation yekuunganidza ropa chubhu;

☛Masero emuenzaniso: Masero akaiswa lysed ne cell lysis solution uye ozochengetwa mu liquid nitrogen kana -80°C mufiriji.

4. Kuunganidzwa uye kuchengetwa kweELISA, radioimmunoassay, uye biochemical test samples:

☛Serum (plasma) sampuli: Tora ropa rose woriisa kune procoagulation chubhu (anticoagulation chubhu), centrifuge pa2500 rpm kwemaminitsi angangoita 20, unganidza supernatant, worichengeta munitrogen yemvura kana mu -80°C mufiriji;

☛Weti sampuli: centrifuge sampuli pa 2500 rpm kweanenge maminitsi makumi maviri, uye uichengete mu nitrogen yemvura kana -80 ° C mufiriji;tarisa nzira iyi ye thoracic uye ascites fluid, cerebrospinal fluid, uye alveolar lavage fluid;

☛Sero samples: Paunenge uchiona zvikamu zvakavanzwa, centrifuge masampuli pa2500 rpm kweanenge maminitsi makumi maviri uye uzvichengete mu nitrogen yemvura kana -80 ° C mufiriji;pakuona intracellular components, Dilute the cell suspension nePBS uye kuomesa uye kunyunguduka kakawanda kuparadza masero uye kusunungura intracellular components.Centrifuge pa2500 rpm kweanenge maminetsi makumi maviri uye unganidza iyo supernatant sepamusoro;

☛Mienzaniso yeTissue: Mushure mekucheka zvienzaniso, zviyera uye zviomese mumvura ine nitrogen kana -80°C mufiriji kuti ishandiswe gare gare.

5. Metabolomics specimen collection:

☛Weti sampuli: Centrifuge sampuli pa 2500 rpm kweanenge maminitsi makumi maviri uye uichengete mu nitrogen yemvura kana -80 ° C mufiriji;tarisa nzira iyi ye thoracic uye ascites fluid, cerebrospinal fluid, alveolar lavage fluid, nezvimwewo;

☛Mushure mekucheka sampuli yetishu, zviyera uye woiomesa munitrogen yemvura kana -80 ° C firiji kuti ishandiswe gare gare;