Kuchenjerera kushandiswa kwePetri ndiro
Kuchenesa kwePetri ndiro
1. Kunyura: Soka zvitsva kana zvakashandiswa glassware nemvura yakachena kupfava uye kunyungudutsa zvakabatanidzwa.Usati washandisa glassware itsva, ingoikwesha nemvura yepombi, wozoinyika mu5% hydrochloric acid usiku hwose;Iyo inoshandiswa glassware inowanzo ine mapuroteni akawanda nemafuta, izvo zvisiri nyore kutsvaira mushure mekuoma, saka inofanira kunyudzwa mumvura yakachena pakarepo mushure mekushandiswa kwekutsvaira.
2. Kukwesha: isa girazi rakanyoroveswa mumvura yekuchenesa uye bhurasha kakawanda nebhurashi rakapfava.Usasiye makona akafa uye kudzivirira kukanganisa kwepamusoro pekupedzisira kwemidziyo.Shamba uye woomesa girazi rakacheneswa kuitira pickling.
3. Pickling: Pickling ndeyekunyorovesa midziyo iri pamusoro mumushonga wekuchenesa, unozivikanwawo se acid solution, kubvisa zvinogona kusara pamusoro pemidziyo kuburikidza neoxidation yakasimba ye acid solution.Kunhonga hakufaniri kudarika maawa matanhatu, kazhinji usiku hwose kana kupfuura.Ngwarira pakuisa nekutora midziyo.
4. Flushing: Midziyo mushure mekukwesha uye kunhonga inofanira kunyatsogezwa nemvura.Kunyangwe midziyo yakashambidzwa yakachena mushure mekunhonga inokanganisa zvakananga kubudirira kana kutadza kwesero tsika.Nekugezeswa kwemaoko emidziyo yakakanyiwa, mudziyo wega wega unozodzokororwa "kuzadzwa nemvura - kudururwa" kanenge ka15, uye pakupedzisira kushambidzwa kwe2-3 nguva nemvura inopisa, yakaomeswa kana yakaomeswa, uye yakarongedzerwa kumira.
5. Zvidiro zvepurasitiki zvinoraswa zvinowanzovharwa nemwaranzi kana kucheneswa nekemikari pazvinobuda mufekitari.
Kurongeka kwePetri ndiro
2. Inogona kukamurwa kuita mapurasitiki petri ndiro uye girazi petri ndiro maererano neyakasiyana yekugadzira zvinhu, asi zvese zviri kunze kwenyika petri ndiro uye kuraswa petri ndiro zvinhu zvepurasitiki.
3. Maererano nehukuru hwakasiyana, vanogona kuparadzaniswa kuva 35mm, 60mm uye 90mm muhupamhi.150mm Petri ndiro.
4. Maererano nezvikamu zvakasiyana, zvinogona kugoverwa mu 2 yakasiyana Petri ndiro, 3 yakasiyana Petri ndiro, nezvimwe.
5. Zvishandiso zvetsika ndiro zvakakamurwa kuva mapoka maviri, kunyanya mapurasitiki negirazi.Girazi rinogona kushandiswa kugadzira zvigadzirwa, microbial tsika uye inoteedzera tsika yemasero emhuka.Zvigadzirwa zvepurasitiki zvinogona kunge zviri zve polyethylene, zvinogona kushandiswa kamwe chete kana kakawanda.Iwo akakodzera murabhoritari inoculation, scribing, uye mabhakitiriya kupatsanurwa oparesheni, uye anogona kushandiswa kurimwa chirimwa zvinhu.
Kuchenjerera kushandiswa kwePetri ndiro
1. Chikafu chetsika chinocheneswa uye chinoiswa disinfected isati yashandiswa.Ingave yakachena kana kwete ine simba guru pabasa, izvo zvinogona kukanganisa pH yetsika yepakati.Kana mamwe makemikari aripo, anotadzisa kukura kwebhakitiriya.
2. Iyo ichangobva kutengwa tsika ndiro dzinofanira kushambidzwa nemvura inopisa kutanga, yozonyudzwa mu 1% kana 2% hydrochloric acid solution kwemaawa akawanda kuti ibvise mahara alkaline zvinhu, uye yosuka kaviri nemvura yakasvibiswa.
3. Kurima mabhakitiriya, shandisa high pressure steam (kazhinji 6.8 * 10 Pa high pressure steam kusvika kune 5th power), chengetedza iyo 30min pa 120 ℃, iomese pakupisa kwekamuri, kana kushandisa kupisa kwakaoma kuti iite stereo, kureva, isa tsika ndiro muchoto, ichengetedze kwe2h pa120 ℃, uye wozouraya mazino ebhakitiriya.
4. Sterilized tsika ndiro inogona kushandiswa inoculation uye kurima.
Nguva yekutumira: Zvita-28-2022