Nhanganyaya kune Common Microbial Culture Media (I)

Nhanganyaya kune Common Microbial Culture Media (I)

Tsika yepakati imhando yemhando yakasanganiswa inovaka muviri matrix yakagadzirwa kubva kune akasiyana zvinhu zvinoenderana nezvinodiwa zvakasiyana siyana hutachiona kukura, iyo inoshandiswa kutsika kana kupatsanura akasiyana tupukanana.Naizvozvo, iyo matrix inovaka muviri inofanira kunge ine chikafu (kusanganisira kabhoni sosi, nitrogen sosi, simba, inorganic munyu, kukura zvinhu) uye mvura inogona kushandiswa netupukanana.Zvichienderana nerudzi rwema microorganisms uye chinangwa chekuyedza, kune marudzi akasiyana uye nzira dzekugadzirira dzetsika media.

Zvimwe zvinozivikanwa zvetsika nemagariro mukuyedza zvinounzwa sezvizvi:

Nutritional agar medium:

Iyo inovaka muviri agar medium inoshandiswa kuparadzira uye tsika yeakajairika mabhakitiriya, yekumisikidza yehuwandu hwehuwandu hwebhakitiriya, kuchengetedza kwemhando dzebhakitiriya uye tsika yakachena.Zvinonyanya kusanganiswa ndezvi: nyama yemombe, yeast extract, peptone, sodium chloride, agar powder, mvura yakasvibiswa.Peptone uye hupfu hwemombe inopa nitrogen, vhitamini, amino acid uye carbon sources, sodium chloride inogona kuchengetedza yakaenzana osmotic pressure, uye agar ndiyo coagulant yetsika yepakati.

Nutritional agar ndiyo inonyanya kukosha mhando yetsika, iyo ine yakawanda yekudya kunodiwa pakukura kwehutachiona.Nutritional agar inogona kushandiswa kune tsika yebhakitiriya tsika.

Blood agar medium:

Ropa agar medium imhando yenyama yemombe inobvisa peptone medium ine defibriinated ropa remhuka (kazhinji ropa retsuro kana ropa regwai).Naizvozvo, mukuwedzera kune zvakasiyana-siyana zvinovaka muviri zvinodiwa pakurima mabhakitiriya, inogona zvakare kupa coenzyme (senge factor V), heme (factor X) uye zvimwe zvakakosha kukura zvinhu.Naizvozvo, midzimu yetsika yeropa inowanzo shandiswa kurima, kupatsanura uye kuchengetedza mamwe pathogenic microorganisms ari kuda chikafu.

Mukuwedzera, ropa agar rinowanzoshandiswa pahemolysis test.Munguva yekukura, mamwe mabhakitiriya anogona kugadzira hemolysin kuputsa uye kunyungudutsa masero matsvuku eropa.Pavanokura pandiro yeropa, zvindori zvehemolytic zvinopenya kana translucent zvinogona kucherechedzwa zvakapoteredza koloni.Iyo pathogenicity yemabhakitiriya mazhinji inoenderana nehemolytic maitiro.Nekuti iyo hemolysin inogadzirwa nemabhakitiriya akasiyana akasiyana, kugona kwehemolytic kwakasiyanawo, uye chiitiko chehemolysis pandiro yeropa chakasiyanawo.Naizvozvo, hemolysis test inowanzoshandiswa kuona mabhakitiriya.

TCBS yepakati:

TCBS ndeye thiosulfate citrate bile munyu sucrose agar medium.Nokuda kwekusarudza kuparadzaniswa kwepathogenic vibrio.Peptone uye mbiriso yakatorwa inoshandiswa seyakakosha chikafu mutsika yepakati kupa nitrogen sosi, kabhoni sosi, mavhitaminzi uye zvimwe zvinokura zvinhu zvinodiwa pakukura kwebhakitiriya;Iyo yakakwirira yesodium chloride inogona kusangana nezvinodiwa zvehalophilic kukura kwe vibrio;Sucrose seyakaviriswa kabhoni sosi;Sodium citrate, yakakwira pH alkaline nharaunda uye sodium thiosulfate inhibisa kukura kwehutachiona hwemukati.Cow bile poda uye sodium thiosulfate inonyanya kutadzisa kukura kwegiremu-positive bacteria.Mukuwedzera, sodium thiosulfate inopawo sulfur sosi.Muhupo hweferric citrate, hydrogen sulfide inogona kuonekwa nebhakitiriya.Kana paine hydrogen sulfide inogadzira mabhakitiriya, tsvina yakasviba inogadzirwa pandiro;Zviratidzo zveTCBS zvepakati ndeye bromocresol yebhuruu uye thymol yebhuruu, ari acid base zviratidzo.Bromocresol yebhuruu chiratidzo cheacid-base ine pH shanduko ye 3.8 (yero) kusvika 5.4 (blue-green).Kune maviri mavara akasiyana-siyana: (1) iyo asidi yakasiyana-siyana pH 1.2 ~ 2.8, kuchinja kubva kune yero kusvika kune tsvuku;(2) Alkali range ipH 8.0 ~ 9.6, ichishanduka kubva kuyero kuenda kublue.

TSA cheese soybean peptone agar medium:

Kuumbwa kweTSA kwakafanana neyekudya agar.Muchiyero chenyika, chinowanzo shandiswa kuyedza kugadzirisa mabhakitiriya mumakamuri akachena (nzvimbo) dzeindasitiri yemishonga.Sarudza nzvimbo yekuedza munzvimbo inoedzwa, vhura ndiro yeTSA uye uiise panzvimbo yekuedza.Masamples anotorwa kana akaiswa kumhepo kweanopfuura 30min kwenguva dzakasiyana, uye obva agadzirwa kuti averenge koloni.Hutsanana hwakasiyana huyero hunoda hukuru hwakasiyana.

Mueller Hinton agar:

MH medium inonzi microbial medium inoshandiswa kuona kurwisana kwehutachiona kune mishonga inorwisa mabhakitiriya.Iyo isingasarudze svikiro umo ma microorganisms akawanda anogona kukura.Mukuwedzera, starch muzvigadzirwa zvinogona kutora chepfu yakasunungurwa nebhakitiriya, saka haizokanganisa migumisiro yekushanda kwemishonga inorwisa mabhakitiriya.Kuumbwa kweMH yepakati kunenge kwakasununguka, iyo inobatsira kupararira kwemishonga inorwisa mabhakitiriya, kuitira kuti iratidze pachena kukura inhibition zone.Muindasitiri yehutano yeChina, MH yepakati inoshandiswawo bvunzo yekunzwa kwezvinodhaka.Paunenge uchiitisa bvunzo yekunzwa kwezvinodhaka kune mamwe mabhakitiriya akakosha, akadai seStreptococcus pneumoniae, 5% ropa regwai uye NAD inogona kuwedzerwa kune yepakati kuti isangane nezvinodiwa zvekudya zvakasiyana.

SS agar:

SS agar inowanzo shandiswa pakusarudza kuparadzaniswa uye tsika yeSalmonella uye Shigella.Inodzivisa mabhakitiriya e-gram-positive, akawanda coliforms uye proteus, asi haibatsiri kukura kwesalmonella;Sodium thiosulfate uye ferric citrate inoshandiswa kuona chizvarwa chehydrogen sulfide, zvichiita kuti nzvimbo yekoloni iite dema;Neutral red ndiyo pH chiratidzo.Asidhi inogadzira shuga inovirisa yakatsvuka, uye shuga isingaviriki haina ruvara.Salmonella haina ruvara uye yakajeka koloni ine kana isina pakati dema, uye Shigella haina ruvara uye yakajeka koloni.