Maitiro ekushandisa iyo cryopreservation chubhu nesainzi uye nemazvo
Kushandiswa kwechubhu ye cryopreservation isainzi, kwete trilogy iri nyore sekuvhura mvura ine nitrogen tank, kuiisa muchubhu ye cryopreservation uye kuvhara mvura yenitrogen tank.Kushandiswa kwesainzi uye kwakaringana kwe cryopreservation tubes kunogona kudzivirira kurasikirwa kwemasampuli uye kuchengetedza kuchengetedzwa kwevaongorori.
Kushandiswa kwechubhu ye cryopreservation isainzi, kwete trilogy iri nyore sekuvhura mvura ine nitrogen tank, kuiisa muchubhu ye cryopreservation uye kuvhara mvura yenitrogen tank.Kushandiswa kwesainzi uye kwakaringana kwe cryopreservation tubes kunogona kudzivirira kurasikirwa kwemasampuli uye kuchengetedza kuchengetedzwa kwevaongorori.
Kutonhora kwechubhu: nhanho dzechando
Geza maseru nepreheated PBS solution, sveta mhinduro, uye uvhare maseru nemhinduro ine trypsin uye EDTA (yakaonda yemvura layer inokwana, uye kusungirirwa kwe trypsin uye EDTA kunoda kutariswa zvinoenderana nesero mutsara).
Isa masero pa 37 ℃ kwemaminitsi 3-5.
Mushure mokunge masero avhara kubva pasi, kusungirirwa kunoguma, iyo yepakati ine serum inowedzerwa, uye masero anorembera zvinyoronyoro nepipette.
Centrifuge iyo cell suspension (500 xg, 5 min) uye kumiswa nepakati ine serum.
Kuwanda kwemasero.
Centrifuge iyo cell suspension (500 xg, 5 maminetsi), bvisa iyo supernatant, uye kusimudzira masero nepakati ine serum yevhoriyamu yakakodzera.
Sanganisa maseru uye cryopreservation solution (60% yepakati, 20% fetal bovine serum, 20% DMSO) muchiyero che1:1 yevhoriyamu, wozoaendesa kuCryo STM cryopreservation chubhu.Kuwanda kwemasero echando ndeye 1-5 × 106 zvidimbu / ml.
Cryo ine masero STM cryopreservation tube inokurudzirwa kutonhora pasi pamwero we - 1 K / min, uye cryopreservation tube inogona kuiswa mumudziyo une isopropanol pa -70 ℃.Kana Cryo STM cryopreservation chubhu ichichengeta mamwe masampuli, anogona kuiswa zvakananga pa - 20 ℃, - 70 ℃ kana gasi chikamu chemvura nitrogen.Kuti ive nechokwadi chekuti sampu yakaomeswa nechando zvakafanana, 4 mL uye 5 mL Cryo Iyo sTM cryopreservation chubhu inoda kuiswa mufiriji pa -20 ℃ kwehusiku humwe, uye yozoendeswa kune gasi chikamu che - 70 ℃ kana liquid nitrogen.
Wobva waendesa Cryo.sTM cryopreservation chubhu kune mvura nitrogen tank.Kuti udzivise kusvibiswa (senge mycoplasma) uye kufunga nezvekuchengetedza, ndapota isa Cryo.sTM cryopreservation chubhu muchikamu chegasi chenitrogen yemvura, kwete muchikamu chemvura.
Maitiro ekushandisa iyo cryopreservation chubhu nesainzi uye nemazvo?Kambani yedu inoumbwa nenyanzvi dzine indasitiri yekumashure uye ruzivo rwakapfuma rwemusika kupa zvigadzirwa zvehupenyu sainzi yekutsvagisa ndima uye masevhisi evaongorori vesainzi.Iyo haigone chete kusangana nezvinodiwa zvakakosha zvevatengi veR&D pamhando dzechigadzirwa uye kurongedza, asi zvakare kusangana nezvinodiwa zvakakwana zvemabhizinesi ekugadzira pamatanho ese kubva padiki, chiyero chepakati kusvika kugadzirwa kwakakura.
Nguva yekutumira: Nov-25-2022