Nzira yekusarudza PCR ndiro yezvigadzirwa zverabhoritari?
PCR mahwendefa anowanzoita 96-gomba uye 384-gomba, achiteverwa ne24-gomba uye 48-gomba.Iyo PCR chiridzwa chakashandiswa uye chimiro chekushandisa chirikuenderera mberi chinoona kana PCR board yakakodzera kuyedza kwako.Saka, ungasarudza sei PCR bhodhi yezvekushandisa murabhoritari nenzira kwayo?
1, Mhando dzakasiyana dzesiketi hadzina masiketi mabhodhi uye dzinoshaya dzakatenderedza mapaneru.
Iri rudzi rwekuita ndiro rinogona kuchinjirwa kune akawanda mamodule ePCR zviridzwa uye chaiyo-nguva PCR zviridzwa, asi haina kukodzera kune otomatiki application.
Iyo hafu-skirt ndiro ine mapfupi mipendero yakatenderedza kumucheto kweplate uye inopa yakakwana rutsigiro panguva yekufambisa kwemvura.Mazhinji eApplied Biosystems PCR zviridzwa zvinoshandisa hafu-skirt mahwendefa.
Iyo yakazara-skirt PCR bhodhi ine mupendero pani inovhara kureba kwebhodhi.Iri rudzi rwebhodhi rakakodzera PCR chiridzwa chine protruding module (iyo inobatsira kune otomatiki kushanda), uye inogona kuve yakachengeteka uye yakadzikama yakagadziriswa.Iyo yakazara siketi zvakare inosimudzira iyo mechanic simba, ichiita kuti ive yakakodzera kwazvo kushandisa irobhoti chikuva mune otomatiki workflow.
2, Mhando dzakasiyana dzepaneru
Iyo yakazara-flat mapaneru dhizaini inoshanda kune akawanda PCR zviridzwa uye yakanakira kuiswa chisimbiso nekugadzirisa.
Iyo yekumucheto convex plate dhizaini ine yakanaka kuchinjika kune zvimwe PCR zviridzwa (seApplied Biosystems PCR chiridzwa), iyo inobatsira kudzikamisa kudzvanywa kwevhavha yekupisa pasina kudiwa kweadapter, kuve nechokwadi chekutamisa kupisa kwakanaka uye mhedzisiro yekuyedza yakavimbika.
PCR mahwendefa anogona kazhinji kupa akasiyana siyana emavara mafomu kuti afambise kusiyanisa kwekuona uye kuzivikanwa kwemasamples, kunyanya mukuedza kwepamusoro.Kunyangwe iwo ruvara rwepurasitiki rusina mhedzisiro pakukwidziridzwa kweDNA, zvinokurudzirwa kushandisa zvigadzirwa zvepurasitiki zvichena kana zvinwiwa zvepurasitiki zvine chando pane zvinobatika pachena paunenge uchiseta maitiro eiyo chaiyo-nguva fluorescence quantitative PCR kuti uwane inonzwisisika uye yakarurama fluorescence kuonekwa.
4. Yakasiyana chamfer nzvimbo
Kucheka kwekona ikona yekushayikwa kwePCR plate, zvinoenderana nechiridzwa chinogadziriswa.Iyo chamfer inogona kunge iri paH1, H12 kana A12 ye96-hole ndiro, kana A24 ye384-hole ndiro.
5, ANSI/SBS fomati
Kuti ienderane neyakasiyana otomatiki mvura inobata yakakwirira-kuburikidza masisitimu, PCR board inofanirwa kutevedzera American National Standards Association (ANSI) neSosaiti yeBiological and Molecular Sciences (SBS), iyo ikozvino yakabatana neLaboratory Automation uye. Screening Association (SLAS).Bhodhi rinoenderana neANSI/SBS rine saizi yakajairwa, kureba, nzvimbo yegomba, nezvimwe, izvo zvinobatsira pakugadzirisa otomatiki.
6, Hole kumucheto
Pane mupendero wakasimudzwa wakapoteredza gomba.Iyi dhizaini inogona kubatsira kuvhara nekuvhara ndiro firimu kudzivirira kubuda.
7, Mako
Kazhinji kacho kanenge kakasimudzwa mucherechedzo wealphanumeric nerunyoro ruchena kana rutema muruvara rwekutanga kuti uonekwe zviri nyore.
Nguva yekutumira: Feb-10-2023