Nzira yekusarudza yakanakisa "Friza chubhu"?

Nzira yekusarudza yakanakisa "Friza chubhu"?

Iyo iri nyore kushandisa cryo chubhu haigone kungozadzisa zvinodiwa zvekuedza, asi zvakare kuderedza mukana wenjodzi dzekuyedza kusvika pamwero wakati.

Nhasi tichashandisa nzira nhatu dzekusarudza cryo chubhu.

Danho rokutanga: zvinhu

Sezvatinoziva tese, machubhu ekuomesa nechando anonyanya kushandiswa pakutakura-kupisa uye kuchengetwa kwematishu kana sero samples, kazhinji mukutsvaga kwebiology uye minda yekurapa.

Nekuda kwekuti chubhu inotonhora yakanangana nesample, danho rekutanga nderekusarudza chinhu chakakodzera kudzivirira kusvibiswa kwemuenzaniso.

Kazhinji, machubhu ekuomesa anogadzirwa nezvinhu zvisina cytotoxicity.Zvinhu zvinowanzoshandiswa mumarabhoritari ipurasitiki negirazi.Zvisinei, nokuti magirazi egirazi haagone kushandiswa pa-high-speed kana overspeed centrifuges, plastiki cryotubes inowanzoshandiswa.

Pane zvakawanda zvepurasitiki zvinhu, ungasarudza sei?

Mashoko mashanu, "polypropylene material" Sarudza nechivimbo!

Polypropylene ine yakanakisa makemikari uye tembiricha kugadzikana.Pasi pegasi mamiriro emvura nitrogen, inokwanisa kumira tembiricha yakaderera kusvika ku minus 187 ℃.

Mukuwedzera, kana zvinodiwa zvekuchengetedzwa kwemuenzaniso zvakakwirira, kwete mutagenic zvinhu uye pyrogen yemahara VID inowirirana tubes inogona kusarudzwa.Uye ndapota usavhura usati washandisa.Kana yakatovhurwa, inofanira kuvharwa isati yashandiswa!

Danho rechipiri: Maumbirwo

Iyo inotonhora chubhu inowanzo umbwa nechubhu chivharo uye muviri wechubhu, iyo yakakamurwa kuita yemukati cap inotonhora chubhu uye yekunze cap inotonhora chubhu.Kana sampuli ichizochengetwa mumvura yenitrogen phase, shandisa mukati kutenderera inotonhora chubhu ine silica gel pad;Kana sampuli ichizochengetwa mumichina yemuchina, sefiriji, iyo yekunze inotenderera inotonhora chubhu inoshandiswa, kazhinji isina silica gel pad.

Nezwi rimwe chete:

Pakazara, kushomeka kwekushisa kwemukati kwemukati kutenderera cryopreservation chubhu iri nani pane iyo yekunze kutenderera inotonhora chubhu, iyo inofanirwa kusarudzwa zvinoenderana nezvinodiwa chaizvo.

Danho rechitatu: tsanangudzo

Zvinoenderana nezvinodiwa zvekuyedza, machubhu e-cryopreservation anowanzo aine 0.5ml, 1.0ml, 2.0ml, 5ml, nezvimwe.

Iyo inowanzoshandiswa biological sample yechando chubhu inowanzoita 2ml muhukuru.Izvo zvinofanirwa kucherechedzwa kuti vhoriyamu yemuenzaniso kazhinji haigone kudarika zvikamu zviviri kubva muzvitatu zvevhoriyamu yechubhu inotonhora.Naizvozvo, iyo yakakodzera yechando chubhu inofanirwa kusarudzwa zvinoenderana nehukuru hweiyo sampuli yechando

Pamusoro pezvo, pane misiyano pakati pekaviri layer uye isina kaviri layer, inogona kugadzwa uye haigone kugadzwa, yemumba uye kunze kwenyika, uye mutengo.Izvi ndizvo zvinhu zvinoda kutariswa pakusarudza chubhu inotonhora.