Nzira yekusarudza sei kushandiswa kwesero tsika "flasks, ndiro uye ndiro"?

Nzira yekusarudza sei kushandiswa kwesero tsika "flasks, ndiro uye ndiro"?

Pakurima maseru, nguva yekushandisa maflasks etsika uye nguva yekushandisa mabhodhoro ndiro zvinoenderana nechinangwa uye zvinodiwa zvekuyedza.Kazhinji, masero etsika maflask anoshandiswa kwekutanga masero etsika uye akajairwa subculture, uye nhamba huru yemasero ekuedza anogona kuwanikwa.

Mafurasiki etsika dzemasero akagadzirwa emhando yepamusoro polystyrene (PS) zvinhu, anogadzirwa neakanyanya-chaiyo mold uye azere otomatiki ekugadzira maitiro.Izvo zvigadzirwa zvinoshandiswa mu laboratori cell tsika.Iwo akanakisa optical zvimiro zvinogonesa kucherechedzwa kwemicroscopic.Iyo yepamusoro yakabatwa neTC kuve nechokwadi chekunamatira sero.Mibairo iri nani.

1) Maitiro ekusarudza maflask etsika uye tsika mahwendefa kune tsika masero

Chekutanga, sarudza zvichibva pane inotarisirwa goho remasero.

Chechipiri, sarudza zvichibva pahunyanzvi hwekuita zvekuyedza.Kungave kuri kushandura media, nzira, kana kukohwa maseru, kushanda kwemidziyo yetsika kuri nyore, asi nekuda kwekuvhurwa kwayo kukuru, iri nyore kusvibisa.

2) Zviri nani kushandisa sero tsika mahwendefa ekuedza anoshandisa masero sevatakuri kana zvinhu, zvakadai sekuongororwa kwezvinodhaka, MTT (96-well culture plate), immunohistochemistry (6-well culture plate), nezvimwewo.