Wakadzidza here chiyero chekuisa chisimbiso firimu?
Chii?Ndiani mumwe asingakwanisi "kuvhara firimu"?Kurumidza kufunga nezvechinyorwa ichi kuti ikudzidzise iwe chaiyo "sealing film"!
Ehe, iyo "sealing firimu" pano ndeyekusimbisa iyo 96 tsime PCR ndiro kuti ive nechokwadi chekuti firimu rekuvharisa rinonyatsoenderana ne96 hole ndiro uye kudzivirira kubuda kwemvura, kuitira kuve nechokwadi chekuyedza kwakatsetseka.
1. Namira firimu rekuvhara pabhodhi
Bvisa imwe sealing membrane kubva mubhegi rekuzvivharisa, wobva waisa zvakare bhegi rekuzvivharisa kuti uchengetedze nharaunda isina enzyme.Chengetedza mutsara wepasi wakatarisa kumusoro, bata firimu rekuvhara, uye zvishoma nezvishoma bvarura jira rezasi uchitevedza tangent line.
Zvadaro, namatira imwe mugumo wekunamatira pamusoro pefirimu yekuisa chisimbiso pabhodhi, uye ubate chinhambwe uye kona kuti udzivise skew inotevera.Mukuita kunamira, imwe magumo inonamirwa uye imwe inodhonzwa.
Zano: Gara wakapfeka magirovhosi
● Kana iyo firimu yekuvhara ye single end label inoshandiswa, bvisa liner zvishoma, simbisa firimu rekuvhara pabhodhi kuti uisimbise pabhodhi rose, uye ramba uchibvisa liner.Iyi nzira inogona kubvisa curl uye rollback inokonzerwa nekuvhara firimu.
● Kana chigadzirwa chine mavara maviri ekupedzisira chikashandiswa, bvisa pakati liner nenzira inopfuurira uye yakatsetseka.Kubvisa zvishoma nezvishoma jira rinoderedza kukoromoka.Ngwarira kuti usabata chisungo chepamusoro chefirimu.
2. Kudzvanya firimu
Shandisa ndiro yekumanikidza kuti utsvake zvishoma nezvishoma uye wodzvanya firimu rekuvhara ndiro kuti riite rakanamwa zvachose pandiro.Kana pasina maramu akakosha, unogona kuwana kadhi rine mipendero yakatsetseka, senge kadhi rebhangi kana kadhi rebhazi.
Iyo firimu yekudzvanya nhanho ichaitwa kanenge kaviri yakatwasuka uye yakatwasuka.Zvakakosha kushandisa simba rakakwana kuti uwane chisimbiso chakanaka.
Pera uye dzvanya membrane yekudzvanya ndiro pamwe nekunze kwese kwendiro ye orifice kanenge kaviri kuti uone kuti yakasimba uye inoenderera kudzvanywa inoiswa.Maburi nemapendero achatsikiswa kamwe chete.Mushure mekuisa chisimbiso firimu rekuvhara nemazvo pandiro, dhonza chikamu chekubatana pamwe nemutsara we tangent.
Zano: ● Paunenge uchidzvanya firimu, bata bhodhi nerumwe ruoko kuti udzivise kuzunungusa kwebhodhi.
3. Kuongorora
Mushure mekuisa chisimbiso, nyatsotarisa ndiro yakatsetseka kuti uone kana iyo firimu yakanyatsobatanidzwa nendiro.Simbisa kuti mavanga ekunamatira akatenderedza gomba rega rega, nzvimbo yese yeplate (kusanganisira periphery) yakavharwa, uye kana paine mvura pane membrane.Iyo firimu yekuisa chisimbiso haifanirwe kuve nemakwinya.Kana makwinya akaonekwa, ndiro haina kuvharwa nemazvo.
● Pamahwendefa akatsetseka ane mipendero yakasimudzwa, kuiswa kwefirimu yekuisa chisimbiso pandiro inogona kunge isina kururama, uye firimu haifaniri kukwira kumusoro kumadziro emadziro eplate.
Isa ndiro yakavharwa kweinenge 10 maminetsi usati watanga kuedza kwePCR, uye simba rekunamatira rekuvhara firimu rinowedzera nekufamba kwenguva.Kana zvichiita, shandisa special centrifuge ye orifice plate ye centrifugation.Pakupedzisira, endesa ndiro yakavharwa kumuchina wePCR kuti utange kuedza ~
Zano:
● Pamahwendefa akatsetseka ane mipendero yakasimudzwa, kuiswa kwefirimu yekuisa chisimbiso pandiro inogona kunge isina kururama, uye firimu haifaniri kukwira kumusoro kumadziro emadziro eplate.
Isa ndiro yakavharwa kweinenge 10 maminetsi usati watanga kuedza kwePCR, uye simba rekunamatira rekuvhara firimu rinowedzera nekufamba kwenguva.Kana zvichibvira, shandisai special centrifuge ye orifice plate ye centrifugation.Pakupedzisira, endesa ndiro yakavharwa kumuchina wePCR kuti utange kuedza ~
Nguva yekutumira: Zvita-16-2022