A centrifuge chubhu ichubhu yakapfava inokwanisa kushingirira kumhanya kwakakwira nekudzvanywa, sekuparadzanisa mamwe masampuli uye kupatsanura madhiri epamusoro.Iyo ultrafiltration centrifuge chubhu ine zvikamu zviviri zvakafanana nechubhu yemukati uye yekunze chubhu.Iyo yemukati chubhu i membrane ine humwe huremu hwemolecular.Munguva yekumhanya-mhanya kwepakati, avo vane huremu hudiki huremu vanopinda muchubhu yepasi (kureva chubhu yekunze), uye avo vane huremu hwakakura vanozovharirwa muchubhu yekumusoro (kureva chubhu yemukati).Iyi ndiyo nheyo ye ultrafiltration uye inowanzoshandiswa kuisa pfungwa pasampuli.
Ultrafiltration centrifuge tubes kazhinji inogona kushandiswa pasina pretreatment, asi yeprotein sample processing, kunyanya kune dilute protein solutions (<10ug / ml), chiyero chekudzoreredza chekutarisa ne ultrafiltration membranes kazhinji haina kuwanda.Kunyangwe zvishandiswa zvePES zvichideredza kushambadza kusingatarisike, mamwe mapuroteni, kunyanya kana akanyungudika, anogona kunge aine matambudziko.Chiyero chekusungirirwa chisiri chakananga chinosiyana nechimiro cheprotein yega.Mapuroteni ane machaji kana hydrophobic domains anowanzo sunga zvisingadzoreki kunzvimbo dzakasiyana.Passivation pretreatment pamusoro peiyo ultrafiltration centrifuge chubhu inogona kuderedza kurasikirwa kweprotein adsorption pane membrane pamusoro.Muzviitiko zvakawanda, pretreatment yekoramu isati yaisa iyo dilute protein solution inogona kuvandudza mwero wekudzoreredza, nekuti mhinduro inogona kuzadza isina mapuroteni adsorption nzvimbo dzakafumurwa pa membrane uye pamusoro.Iyo passivation nzira ndeyekutanga kunyudza iyo column ine yakakwira vhoriyamu yepassivation mhinduro kweawa inopfuura 1, geza iyo mbiru zvakakwana nemvura yakashambwa, uye wozoisa centrifuge kamwe chete nemvura yakanyungudutswa kuti ubvise zvachose passivation mhinduro inogona kuramba iri pafirimu. .Chenjerera kuti usarega iyo firimu yakaoma mushure mekupfuura.Kana iwe uchida kuishandisa gare gare, unofanirwa kuwedzera mvura isina kuchena yakasvibiswa kuti firimu rirambe rakanyorova.
Ultrafiltration centrifuge chubhu haigone kuvharirwa uye kushandiswa zvakare.Sezvo mutengo wechubhu imwe chete isingadhuri, vanhu vazhinji vanoedza kuishandisa zvakare - chiitiko ndechekuchenesa membrane pamusoro nemvura yakasvibiswa kwenguva dzakawanda uye centrifuge iyo kamwe kana kaviri.Iyo diki chubhu inogona kukwiridzirwa kumashure inogona kunyudzwa mumvura yakanyungudutswa uye yobva yaiswa centrifuged in reverse kwenguva dzakawanda, zvinova zviri nani.Inogona kushandiswa kumuenzaniso mumwechete kakawanda, uye inogona kunyudzwa mumvura yakasvibiswa kana isiri kushandiswa, asi kusvibiswa kwebhakitiriya kunodzivirirwa.Usasanganise masampuli akasiyana.Vamwe vanhu vanoti kunyura mu20% doro uye 1n NaOH (sodium hydroxide) kunogona kudzivirira kukura kwebhakitiriya uye kudzivirira kuoma.Chero bedzi iyo ultrafiltration membrane inopinda mumvura, haigoni kubvumirwa kuoma.Zvisinei, vamwe vanoti ichaparadza membrane chimiro.Chero zvazvingava, vagadziri kazhinji havatsigire kushandiswazve.Kudzokororwa kushandiswa kunovharira saizi yepore yesefa membrane, uye kunyange kukonzera kubuda kwemvura, izvo zvinokanganisa mhedzisiro yekuyedza.
Nguva yekutumira: Sep-05-2022